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This quantity in a research-level sequence covers different features of microbial body structure and biochemistry together with inositol metabolisms in yeasts, bacterial adhesion, natural acids, the bacterial flagellum and the mechanical behaviour of bacterial telephone partitions. it really is meant to be of use to microbiologists, biochemists and biotechnologists. different similar works during this sequence are volumes 29, 30 and 31.
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Extra resources for Advances in Microbial Physiology, Vol. 21
1979). In uitro transcription by chromatin, or nuclei, isolated during the S-phase, where transcription by polymerase I1 predominates, can be strongly stimulated by the addition of exogenous, homologous, polymerase 11. In contrast, the stimulation by exogenous enzyme is very much less with chromatin or nuclei from the GZ-phase. This observation tentatively suggests that genes which are to be transcribed by polymerase I1 are inaccessible to the enzyme during the GP-phase, or else that inhibitors are produced during the cell cycle which selectively inactivate one or the other of the two polymerases and which would also inactivate, in GP-phase TRANSCRIPTION IN ACELLUIAR SLIME MOULDS 33 chromatin, the exogenous polymerase.
P. Biochemistry, New York I%, 2334. Holt, C. E. (1979). In “Growth and Diferentiation in Physarum polycephalum” (W. F. Dove and H. P. ). Princeton University Press, in press. 44 T. SEEBECK AND R. BRAUN Howard, F. L. (1932). Annals ofBotany 46,461. Hiittermann, A. (1973). ” Gustav Fischer Verlag, Stuttgart. S ~ C342. S Jacobson, D. N. and Halt, C. E. (1973). Archives ofBiochemistry and B ~ O P ~ Y 1599 James, G. , Yeoman, L. , Matsui, S. , Goldberg, A. H. and Buschi H. Eiochemdv, New Yorh 16, 2384.
This is sufficient to completely inhibit all RNA polymerase I found in this number of nuclei when assayed in uitro. During maturation of the spherules the inhibitor concentration decreases and in mature spherules its concentration has dropped to undetectable levels (Hildebrandt and Sauer, 197 7a). B. The activity of this factor (measured as stimulation of RNA polymerase I1 activity in vitro) gradually decreases during starvation to undetectable levels. From the evidence presently available it is difficult to judge if its activity, and hence its disappearance, is of consequence for one o r othei- o r both of the two RNA po lymerases.