Download Advances in Microbial Physiology, Volume 19 by A.H. Rose (Editor), J. Gareth Morris (Editor) PDF

By A.H. Rose (Editor), J. Gareth Morris (Editor)

This quantity in a research-level sequence covers different features of microbial body structure and biochemistry together with inositol metabolisms in yeasts, bacterial adhesion, natural acids, the bacterial flagellum and the mechanical behaviour of bacterial mobilephone partitions. it really is meant to be of use to microbiologists, biochemists and biotechnologists. different comparable works during this sequence are volumes 29, 30 and 31.

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Data would strongly support the idea that these strains have only a single genetic lesion, and they grow at exactly the same rate as the isogenic wild-type in five different media. Very long chains of unseparated bacilli are formed in media allowing fast growth rates (see Fig. 6) and shorter ones in those giving slow growth rates. This would be predicted from Paulton’s (1970) calculations assuming a greatly prolonged separation time for the individual bacilli. Walls of the mutant bacteria were as readily lysed as those from the wild-type by autolytic enzyme preparations made from the latter, and their chemical composition was in no detectable way abnormal.

3. Round mutants from Escherchia coli Mutants of E. , 1974). Those which have been examined have peptidoglycans that do not differ from the wild-type; many if not all are more sensitive to lysis by neutral detergents than the wild-type, possibly indicating some abnormality in the outer membrane. Apart from these fairly general properties, the physiological phenotypes of the various mutants would seem to show considerable diversity. , 1972). Magnesium ions were necessary, and growth was improved by the presence of 12% sucrose.

1969) grew as very large irregular clumps. , 1976). In all observations recorded, the growth rate of autolyticdeficient strains is similar to, or identical with, that of the wild-type despite the loss of up to 95% of autolytic activity. This gives little support to any essential role of autolysins in growth of cells. Moreover, since the two known autolytic enzymes in staphylococci are an N-acetylmuramylL-alanine amidase and an endo-j-N-acetylglucosaminidase,it would be difficult to propose a role for them in biogenesis of walls.

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