Download Affinity Labeling: Affinity Labeling by Nathan P. Kaplan, William B. Jakoby, Meir Wilchek PDF

By Nathan P. Kaplan, William B. Jakoby, Meir Wilchek

The severely acclaimed laboratory general, equipment in Enzymology, is likely one of the such a lot hugely revered guides within the box of biochemistry. on the grounds that 1955, every one quantity has been eagerly awaited, usually consulted, and praised via researchers and reviewers alike. The sequence comprises a lot fabric nonetheless suitable this day - really a necessary book for researchers in all fields of existence sciences.

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Lane, and M. C. Scrutton, J. Biol. Chem. 245, 4939 (1970). aa K. D. Collins, J. Biol. Chem. 249, 136 (1974). T. G. Spring and F. Wold, Biochemistry 10, 4655 (1971). cc G. J. Cardinale and R. H. Abeles, Bioehemislry 7, 397 (1968). dd S. Wang, F. S. Kawahara, and P. Talalay, J. Biol. Chem. 238, 576 (1973). ee R. Wolfenden, Biochemistry 9, 3404 (1970). / / J . M. Chirgwin and E. A. Noltmann, Fed. Proc. 32, 667 (1973). gg D. Cassio, F. LeMoine, J. P. Waller, E. Sandrin, and R. A. Boissonas, Biochemistry 6, 827 (1967).

R. Rando, Nature (London) 250, 586 (1974); R. 1t. Rando, Biochemistry 13, 3859 (1974); R. R. Rando, N. Relyea, and L. Chang, J. Biol. Chem. 251, 3306 (1976). s I. Giovanelli, L. D. Owens, and S. H. Mudd, Biochim. Biophys. Acta 227, 671 (1971). E. W. Miles, Biochem. Biophys. Res. Commun. 66, 94 (1975). 32 ~] GENERAL METHODOLOGY tryptophan synthetase by 2, and ~-cystathionase by 3. The inhibition of aspartate aminotransferase by 1 and ~ involves the sequence of steps shown in Eq. (4). j *~N/°~. ~ H H (4) when R--OCH3 (4) -- OCH~ Enz ~ e ~ e f C O 2" CHa I I ÷ II tt Since both inhibitors must be converted into the active form 4 by the enzyme, neither one inhibits the holoenzyme in the pyridoxamine form or the apoenzyme.

Cooper, Biochem. Biophys. Res. Commun. 23, 243 (1966); T. G. Rajogopalan, W. H. Stein, and S. Moore, J. Biol. Chem. 241, 4295 (1966). lJ M. Brockhaus and J. Lehmann, FEBS Lett. 62, 154 (1976). 'ell. Chaimovich, R. J. Vaughan, and F. H. Westheimer, J. Am. Chem. Soe. 90, 4088 (1968) ; J. Sharer, P. Baronowsky, R. Lawsen, F. Finn, and F. H. Westheimer, J. Biol. Chem. 241, 421 (1966). [3] 37 MECHANISM-BASED IRREVERSIBLE INHIBITORS These compounds are thought to function by undergoing elimination reaction with certain enzymes to give rise to reactive Michael acceptors.

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